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KMID : 1007520130220020573
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Food Science and Biotechnology
2013 Volume.22 No. 2 p.573 ~ p.576
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Clone and Identification of Bifunctional Δ12/Δ15 Fatty Acid Desaturase LKFAD15 from Lipomyces kononenkoae
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Yan Zhang
Zhuo Liang
Mulan Jiang
Xia Wan
Gon Yangmin
Yinbo Zhang
Huang Fenghong
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Abstract
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In modern diet of human being, the ω-6/ω-3 ratio of dietary fatty acid was shifted much towards ω-6 series. In this work, a fatty acid desaturase (FAD) gene lkfad15 was cloned and identified from oleaginous yeast Lipomyces kononenkoae. Function analysis results shown that LKFAD15 is a novel Δ12/Δ15 bifunctional FAD which could not only produce linoleic acid and α-linolenic acid use just oleic acid as substrate but also adjust the ω- 6/ω-3 fatty acids ratio to the WHO recommended ratio. Phylogenetic analysis of LKFAD15 suggested it is a specific intermediate product of gene evolution derives from independent gene duplication events.
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KEYWORD
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Lipomyces kononenkoae, fatty acid desaturase, polyunsaturated fatty acid, α-linolenic acid, linoleic acid
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